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. 2010 Jun 14;189(6):945–954. doi: 10.1083/jcb.201001024

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© 2010 Lacroix et al.

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Figure 5. — Effects of differential microtubule glutamylation on katanin p60. (A) U2OS cells were cotransfected with katanin p60-EYFP and either active TTLL6 or an inactive mutant (TTLL6d), with CFP as transfection control. Activation of katanin leads to MT localization of katanin p60, and sometimes to MT bundling and/or severing. Bar, 20 µm. (B) Fraction of cells showing colocalization of katanin p60-EYFP with MTs (all phenotypes as shown in A were compiled). A minimum of 200 cells was analyzed in three independent experiments. Error bars indicate SD.