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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Bioorg Med Chem Lett. 2009 Dec 4;20(2):444–447. doi: 10.1016/j.bmcl.2009.12.001

Figure 2.

Figure 2

A representative plot for the disappearance of 2 in the presence of thiol. Compound 2 (2.5 µL of a 10 mM stock in CH3CN) was added to a mixture containing sodium phosphate buffer (50 µL, 500 mM, pH 7.0), water (150 µL), 2-mercaptoethanol (50 µL of a 10 mM stock) and acetonitrile (247.5 µL) at 25 °C. The mixture (final concentrations: 2, 50 µM; buffer, 50 mM, pH 7.0; thiol, 1 mM; acetonitrile, 50% by volume) was vortex mixed and the disappearance of 2 (a is the peak area at time = t and a0 is the peak area at time = 0) monitored by reverse phase HPLC at regular time intervals as described in the legend for Figure 1. From the slope of the plot, a pseudo-first-order rate constant of 5.5 × 10−3 s−1 (t1/2 = 2 min) at 1 mM thiol was obtained. This corresponds to an apparent second-order rate constant of 5.5 ± 0.2 M−1 s−1.