Table 3.
Reaction flux and related parameter values at resting steady state, in mmol/min
| ϕ0 | Vmax,x,S↔Pf | Vmax,x,S↔Pb | Km,S↔Pf | Km,S↔Pb | Kγ | ηx,S↔P0 | |
|---|---|---|---|---|---|---|---|
| Hexokinase | 0.195 | 0.59 | 4.34E-12 | 3.29 | 3.93E-07 | 27.8 | 21 |
| Glycogen synthase | 0.250 | 27.777 | 9.45E-07 | 17400 | 158 | 0 | 1.8 |
| Glycogen phosphorylase | 0.250 | 0.254 | 3.50E+6 | 0.00306 | 293000 | 1.87e-5 | 1 |
| Phosphoglucose isomerase | 0.195 | 1.126 | 5.37E-01 | 0.278 | 0.0486 | 0 | 4.12 |
| Phosphofructokinase | 0.195 | 7.852 | 2.49E-10 | 0.288 | 9.42E-08 | 0.841 | 4.12 |
| Aldolase +TPI | 0.195 | 2.670 | 3.30E-01 | 0.667 | 0.079 | 0 | 4.12 |
| GA3P dehydrogenase | 0.382 | 1.146 | 7.37E-08 | 0.0387 | 1.96E-09 | 0 | 4.12 |
| Phosphoglycerate kinase | 0.382 | 1.192 | 4.09E-02 | 0.00158 | 0.0319 | 0 | 4.12 |
| Pyruvate kinase | 0.382 | 1.171 | 3.21E-02 | 7.61E-09 | 0.313 | 0.00267 | 4.12 |
| Lactate dehydrogenase | 0.090 | 9.76E +44 | 7.20E-03 | 1.17E +23 | 1.3E-15 | 0 | 1 |
| Lipases | 0.008 | 0.024 | 7.43E-19 | 16.7 | 4.52E-15 | 0 | 1 |
| Gr3P dehydrogenase | 0.008 | 0.025 | 9.35E-04 | 1.96E-09 | 0.0251 | 0 | 1.2 |
| Acyltransferase | 0.008 | 0.024 | 2.34E-06 | 8.42E-09 | 0.000273 | 0 | 1.2 |
| Acyl-CoA synthetase | 0.106 | 0.318 | 1.69E-19 | 0.312 | 6.21E-14 | 0 | 1 |
| ATPase | 12.352 | 29.562 | 3.52E-11 | 5.92 | 1.04E-5 | 0 | 1.3 |
| Adenylate kinase | 0 | 200 | 4.01E-02 | 132 | 3.18E-5 | 0 | 1 |
| Creatine kinase | 0 | 200 | 6.65E +0 | 2.97E-4 | 20.7 | 0 | 1 |
| Alanine aminotransferase | 0.065 | 0.197 | 1.83E-03 | 0.0528 | 1.44 | 0 | 1.25 |
| PDH | 0.239 | 0.870 | 8.21E-12 | 0.00315 | 3.81e-7 | 0.361 | 32 |
| β-Oxidation | 0.0819 | 0.164 | 8.8E-189 | 4.67E-4 | 6E-6 | 0 | 1 |
| Citrate synthase | 0.8946 | 6.454 | 2.20E-09 | 0.00048 | 9.55E-7 | 0.361 | 2.25 |
| Isocitrate dehydrogenase | 0.886 | 6.433 | 1.19E-01 | 2.99 | 0.0948 | 0.361 | 2.25 |
| AKG dehydrogenase | 0.8856 | 2.663 | 2.28E-05 | 0.0158 | 0.948 | 0 | 2.25 |
| SCoA synthetase | 0.8856 | 2.687 | 2.69E-02 | 13.5 | 0.33 | 0 | 2.25 |
| Succinate dehydrogenase | 0.8856 | 1.814 | 0.0127 | 2.01 | 0.228 | 0 | 2.25 |
| Malate dehydrogenase | 0.8946 | 29.524 | 2.285E-5 | 2.99 | 3.00E-07 | 0 | 2.25 |
| Complex I +III+IV | 3.753 | 1.126E +32 | 1.75E-0 | 2.05E-6 | 3.15 | 0 | 1.1 |
| Complex II+III+IV | 1.459 | 1.74E +19 | 9.23E-07 | 0.0394 | 2.12 | 0 | 1.1 |
| ATP synthase | 11.573 | 1.92E-08 | 3.76E +0 | 0.000271 | 8.68 | 0 | 1.2 |
| Citrate lyase | 0.00894 | 0.0268 | 2.35E-07 | 0.00557 | 4.89E-08 | 0 | 1.2 |
| ACC | 0.00894 | 0.0537 | 5.96E-13 | 0.00739 | 2.72E-05 | 5.278E-2 | 1.2 |
| Malate dehydrogenase | 0.00894 | 0.0286 | 1.8E-03 | 2.35E-11 | 0.015 | 0 | 1.2 |
| Malonyl-CoA utilization | 0.00894 | 0.0439 | 1.71E-02 | 5.27E-48 | 0.0125 | 0 | 1.2 |
Value of reaction fluxes is based on flux balance analysis. Michaelis-Menten parameters (Kmγf, KmbKγ) are the same as in our previous model (21). Maximal reaction rate coefficients, Vmax are evaluated from the governing equation of reaction flux. Activation coefficients η are optimized to fit the experimental data based on model variation M.3.