Table 2.
Genotype | WT | KO | WT | KO | WT | KO | WT | KO |
---|---|---|---|---|---|---|---|---|
Sex | M | M | M | M | F | F | F | F |
Diet | SC | SC | WD | WD | SC | SC | WD | WD |
Stool lipid extract | 3.08 | 2.31 | 3.68 | 3.26 | 2.19 | 2.19 | 1.81 | 1.43 |
SE | 0.24 | 0.15 | 0.45 | 0.21 | 0.20 | 0.26 | 0.12 | 0.17 |
P WT vs. KO | 0.02 | 0.42 | 0.98 | 0.09 | ||||
n | 8 | 8 | 12 | 12 | 8 | 8 | 12 | 12 |
Stool weight | 3.38 | 3.97 | 1.28 | 1.32 | 3.12 | 3.48 | 0.75 | 1.06 |
SE | 0.43 | 0.26 | 0.14 | 0.10 | 0.40 | 0.09 | 0.26 | 0.10 |
P WT vs. KO | 0.30 | 0.83 | 0.43 | 0.33 |
Male or female iPLA2γ-KO and WT mice were fed SC or WD as in Table 1. Individually housed mice age 12 wk had unrestricted access to food and water, and all stool was collected for 24 h intervals. Stool wet (expressed in grams and tabulated) and dry weights and extractable lipid content (expressed in mg lipid per 60 mg stool dry weight) were determined as in experimental procedures and elsewhere (35, 72).