Table 2.
Effect of dietary iron content on glucose tolerance in ob/ob or wild-type C57BL6 mice eating normal or high-fat chow
| Normal Chow, mg/kg iron |
High-Fat Chow, mg/kg iron |
||||
|---|---|---|---|---|---|
| 35 | 350 | 35 | 350 | 500 | |
| Ob/ob | |||||
| HOMA-B | 808 ± 71 | 567 ± 38* | |||
| HOMA-IR | 77.0 ± 4.9 | 68.0 ± 11.1 | |||
| AUCG, g•min−1•dl | 37.1 ± 2.8 | 40.0 ± 1.9 | |||
| Weight, g | 57.4 ± 1.2 | 58.7 ± 1.7 | |||
| C57BL6 | |||||
| HOMA-B | 76.0 ± 4.8 | 67.4 ± 3.6 | 133.5 ± 21.6† | 59.6 ± 1.9* | |
| HOMA-IR | 3.9 ± 0.5 | 2.9 ± 0.4 | 19.9 ± 4.6† | 8.9 ± 1.5† | |
| AUCG, g•min−1•dl | 27.3 ± 1.4 | 34.2 ± 2.2* | 44.4 ± 4.8† | 42.5 ± 2.3† | |
| Weight, g | 28.9 ± 0.4 | 25.5 ± 1.6 | 40.6 ± 2.4† | 36.7 ± 1.2† | |
| Triglycerides, mg/dl | 38.4 ± 1.9 | 42.8 ± 1.8 | ND | ND | |
| Islet insulin, mg/islet | ND | ND | 0.79 ± 0.07 | 0.50 ± 0.06* | |
Results are means ± SE; n = 10 (ob/ob) or 5 (C57BL6) mice/group. HOMA-B, homeostasis model assessment of β-cell function; HOMA-IR, homeostasis model assessment of insulin resistance; AUCG, area under the glucose curve. At 30 days of age, mice were placed on the indicated chows: normal chow (low fat) or high-fat chow containing the indicated concentrations of iron. After 45 days the mice were weighed, and glucose tolerance testing was performed.
*
P < 0.05 for 35 compared with 350 mg/kg chow within types of chow.
†
P < 0.05 for high-fat compared with normal chow of the same iron content.