. Author manuscript; available in PMC: 2010 Jun 16.

Published in final edited form as: Acta Oncol. 2007 Sep 24;47(3):406–412. doi: 10.1080/02841860701592400

Table 1.

Assay methods used.

		Range of coefficient of variation (%)
Analyte	Method	Intra assay	Inter assay
Estradiol	ALPCO Diagnostics Estradiol EIA	7.5–12.2	8.2–12.1
Progesterone	R&D Systems’ Progesterone EIA	4.9–7.6	2.7–8.3
Testosterone	DPC IMMULITE 2000 Total testosterone Chemiluminescent EIA	5.1–16.3	7.2–24.3
DHEAS	DPC IMMULITE 2000 DHEA-S0₄ Chemiluminescent EIA	4.9–9.8	7.9–13
SHBG	DPC IMMULITE 2000 SHBG Chemiluminescent EIA	0.03–4.2	4.0–6.6
hCG	DPC hCG Coat-A-Count IRMA	2.6–5.8	4.6–7.0
AFP	DPC IMMULITE 2000 AFP Chemiluminescent ELA	2.1–6.3	4.5–12
hPGH	BIOCODE-HYCEL hPGH IRMA	3.0–5.5	5.0–7.9
IGF-1	DSL IGF-1 ELISA (DSL-10-2800)	4.5–8.6	3.3–6.8
IGFBP-3	DSL IGFBP-3 ELISA (DSL-10-6600)	7.3–9.6	8.2–11.4
total IGFBP-1	DSL Total IGFBP-1 ELISA (DSL-10-7800)	1.7–4.6	6.2–7.6
np IGFBP-1	Medix Biochemica IGFBP-1 IEMA	4.4–5.4	4.4–5.9

Note: EIA-enzyme immunoassay; IRMA-immunoradiometric assay; ELISA-enzyme-linked immunosorbent assay; IEMA-immunoenzymetric assay.