Fig. 7.

Caveolin-1 scaffolding domain (CSD) inhibits ERK phosphorylation and SP-C gene expression. A: fluorescence immunocytochemistry images demonstrating uptake of the rhodamine-labeled CSD peptide by type II cells after 6 h of incubation (1 μM). Original magnification ×20. B and C: E19 monolayers were incubated for 6 h with vehicle (veh) or scrambled (scr) peptide or CSD peptide (both at 1 μM) and then exposed to 5% cyclic stretch for 15 min (B) or 16 h (C). Proteins and RNA were collected and processed to detect ERK activation or SP-C gene expression. *P < 0.05 vs. control vehicle or scrambled; **P < 0.05 vs. stretch vehicle or scrambled (n = 4). Blots in B represent results from 4 independent experiments.