Fig. 3.

Zinc deficiency increases NF-κB DNA-binding activity in vivo in the lung following CLP. DNA-binding activity of the transcriptional factor NF-κB p65 was quantified in mouse lung tissue obtained from adult male C57BL/6 mice placed on zinc-modified diets at 6 and 24 h following CLP. Values (means ± SD) were normalized according to cellular protein content. Data are representative of 3 separate experiments (n = 5 animals per treatment group: N, zinc-normal diet; D, zinc-deficient diet; S, short-term zinc supplementation diet after a zinc-deficient regimen). *Statistically significant difference between Zn⁻/CLP and Ctrl/CLP, P < 0.05; ‡statistically significant difference between Zn⁺/CLP and Zn⁻/CLP, P < 0.05.