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. 2010 Apr 2;298(6):L880–L887. doi: 10.1152/ajplung.00384.2009

Fig. 4.

Fig. 4.

3-NT eNOS protein levels (A and B), Ser1177 eNOS protein levels (C and D), and eNOS Thr495 protein levels (E and F) in lung tissue before and 4 h after acute ductal constriction. Acute ductal constriction did not change 3-NT eNOS and Ser1177 eNOS levels in vehicle-treated lambs. However, eNOS Thr495 protein levels, a phosphorylated form of eNOS associated with decreased activity, were increased. Following PEG-SOD treatment, acute ductal constriction did not change 3-NT eNOS levels, but Ser1177 eNOS levels were increased. The increase in eNOS Thr495 levels were not affected by PEG-SOD treatment. In fact, when compared side by side, phospho-Ser1177 eNOS protein levels were greater in the PEG-SOD-treated lambs following ductal constriction than the PEG-alone-treated lambs (G). N = 5 vehicle-treated lambs, n = 7 PEG-SOD-treated lambs. Values are means ± SD. *P < 0.05 vs. Pre.