Fig. 4.

Role of GEF-H1 in CS-induced cytoskeletal rearrangement. Human pulmonary EC were transfected with GEF-H1-specific or nonspecific siRNA. After 72 h of transfection, cells were subjected to 18% CS for various periods of time. A: cytoskeletal remodeling in control and stretched EC monolayers was analyzed by immunofluorescence staining for F-actin. Inset: Western blot analysis of GEF-H1 depletion in HPAEC. B and C: phosphorylation status of MYPT (B) or MLC (C) was determined by Western blot analysis in static and CS-exposed EC transfected with nonspecific or GEF-H1-specific siRNA.