Fig. 5.

Involvement of GEF-H1 in CS-mediated alterations of MT network. Pulmonary EC grown on Flexcell plates were treated with GEF-H1-specific or nonspecific siRNA for 72 h, followed by 18% CS exposure for 0, 30, or 120 min. A: MT structure was analyzed by immunofluorescence staining for β-tubulin. Arrows indicate main direction of CS vector. B: morphometric analysis of assembled MT. Data are expressed as means ± SD of 3 independent experiments; *P < 0.05. C: pools of stable MT were detected by Western blot for acetylated tubulin in samples from static and stretched EC transfected with nonspecific or GEF-H1-specific siRNA.