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. 2010 Mar 26;298(6):L830–L836. doi: 10.1152/ajplung.00014.2010

Fig. 3.

Fig. 3.

Effect of triptolide on proinflammatory gene expression and cell viability. A: dose response for the effect of triptolide on IL-8 production in A549/Tacr1 cells. Cells were treated with the indicated concentrations of triptolide for 30 min and then with 100 nM substance P for 4 h. IL-8 in cell culture supernatants was measured by ELISA. Each point represents mean ± SE for 3 wells per group. B: dose response for the effect of triptolide on NF-κB-luciferase activity in A549/NF-κB-luc cells. Cells were treated with the indicated concentrations of triptolide for 30 min and then with 10 μM protease-activated receptor 2 (PAR-2) agonist (2-furoyl-Leu-Ile-Gly-Arg-Leu-Orn-NH2) for 4 h. Luciferase activity was measured in cellular extracts. Each point represents mean ± SE for 3 wells per group. C: effect of triptolide on cell viability. Cells were treated with XTT and the indicated concentration of triptolide for 4 h, and absorbance of the wells was measured at 450 nm (A450). Values are means ± SE for 8 wells/group.