Skip to main content
. 2010 Apr 2;298(6):H1842–H1849. doi: 10.1152/ajpheart.01236.2009

Fig. 3.

Fig. 3.

Western blot analysis for hERG channels in neonatal mouse cardiomyocytes. Cardiomyocytes transfected with empty pcDNA3 vector (lane 1) have protein bands of 165 and 205 kDa, consistent with mouse ERG1a (mERG1a), and 95 and 114 kDa, consistent with mERG1b (small arrows). Cardiomyocytes transfected with WT hERG (hERG1a; lane 2) have additional protein bands at 135 and 155 kDa (large arrows). Lanes 3 and 4 show G601S hERG and lanes 5 and 6 show N470D hERG without (−) or with (+) E4031 incubation. E4031 incubation resulted in increased density of the 155-kDa bands. Lanes 7 and 8 show ΔY475 hERG without or with E4031 incubation and show both 135- and 155-kDa bands. Lane 9 shows that untransfected cardiomyocytes lack the 135- and 155-kDa bands. Quantitative Western blot density ratio analysis of hERG protein is shown at the bottom. See text for detail.