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Effect of prediabetes on acetylcholine-induced relaxation of isolated resistance arteries (200–300 μm) in the presence and absence of insulin. Vessels were incubated with insulin (1 nM) or its vehicle (saline) for 30 min before administration of acetylcholine (10 μM) to phenylephrine-contracted vessels. White bars represent control rats (n = 11), and black represent prediabetic rats (n = 7). Values are means ± SE. *P < 0.05, different from phenylephrine-induced contraction.
