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. 2010 Apr 2;298(6):H1719–H1726. doi: 10.1152/ajpheart.00937.2009

Fig. 1.

Fig. 1.

Determination of AFos expression in the adult rat ventricular myocytes. A: myocytes were infected with AFos in increasing MOI (50–400 MOI) for Western blot analysis and compared with β-galactosidase (β-Gal; BG) control (C) [100 multiplicity of infection (MOI)]. Anti-hemagglutinin (HA) antibody diluted 1:1,000 shows increasing signal corresponding to increasing expression of AFos. B: myocytes were infected with β-Gal (100 MOI) vs. AFos in increasing MOI. 4,6-Diamino-2-phenolindole (DAPI) stain shows viable myocytes with binuclear expression pattern with corresponding immunofluorescence in rod-shaped myocytes.