A: Akt phosphorylation at phospho (p)-Akt [Ser473 (S473)] and p-Akt [Thr308 (T308)] in WT hearts at R240. Western blot analysis of whole heart lysates collected from WTNT, WTTH, and normothermic WT sham-arrested animals at 240 min. B: densitometry, expressed as a percentage of β-tubulin, revealed significant differences in phosphorylation (P < 0.05, denoted by bracket).