Fig. 5.

Effect of CCPA and SB-203580 on the phosphorylation of heat shock protein 27 (HSP27) in the isolated heart obtained from wild-type and A₁R KO mice. CCPA (1.0 μM) was administered for 5 min in the presence or absence of SB (0.265 μM). Representative film densities are indicated above the corresponding bars for Western blots utilizing general (total HSP27) and phospho-specific antibodies (phospho-HSP27). Density ratios are depicted for each respective bar graph. Values are means ± SE of 4–10 hearts and are the ratio of phosphorylated HSP27 to total HSP27. *Mean is significantly different from the agent-free value (P < 0.05). †Means are significantly different from the wild-type CCPA-alone value (P < 0.05).