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Immunomagnetic isolation of seizure-induced circulating endothelial cells (CECs) on Dynabeads CD31. Mononuclear cells were isolated from peripheral blood collected before seizures (A), 2 h after seizure induction (B), and 4 h after seizure induction by bicuculline (C–E). Shown are representative images of CD31+ cells retained by Dynabeads CD31 (A–C: ×20 objective, D and E: ×100 objective). F: positive control, cultured newborn piglet cerebral microvascular ECs (CMVECs). Bars = 10 μm.
