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Immunophenotyping of cultured ECs from newborn pig cerebral vessels (A–C) and from the aorta (D–F) for CD146, an endothelial antigen (A and D), and GLUT1, the blood-brain barrier-specific GLUT (B and E). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). C and F: merged images for CD146, GLUT1, and DAPI.
