Fig. 1.

Immunofluorescence localization of integrins and the cytoskeleton protein vinculin in adult mouse cardiomyocytes under high confocal magnification (×63). A: immunofluorescence labeling was performed on isolated cardiomyocytes using anti-α₃-integrin with an Oregon green-labeled secondary monoclonal antibody and anti-α₅-integrin with a Cy5-labeled secondary monoclonal antibody. The enlarged inset (right image) shows the overlay of anti-α₃ and anti-α₅-integrin micrographs, where the closest or colocalized areas are indicated as yellow-orange. Regions of costameric (arrows), striated (arrowheads), and nuclear (red asterisks) structures are shown. B: immunofluorescence staining of a single cardiomyocyte using anti-vinculin with an Oregon green-labeled secondary monoclonal antibody and anti-α₅-integrin with a Cy5-labeled secondary monoclonal antibody. The enlarged inset (right image) shows the overlay of anti-vinculin and anti-α₅-integrin micrographs, where the closest or colocalized areas are shown as yellow-orange. Integrin and vinculin exhibited costameric and intercalated disk (blue arrow) localization. Bar = 10 μm.