Fig. 6.

ECFP/ANG II line A TG mice demonstrate abnormal kidney histology. Kidney sections obtained from male mice, 4–6 mo of age, were stained with hematoxylin and eosin (H&E) or periodic acid Schiff (PAS) to evaluate TG kidney phenotype. A: a WT kidney section stained with H&E shows normal kidney structure. B: kidney section from a TG mouse, H&E stained. Glomeruli (Bowman's capsule and intraglomerular space) are enlarged in TG mice. C and D: kidney sections of WT and TG kidneys, respectively, stained with PAS. Note glomerular blood clots consistent with thrombotic microangiopathy. E and F: corresponding sections (C and D, respectively) imaged using fluorescence microscopy. The fusion protein, ECFP/ANG II (ECFP fluorescence in blue) appears primarily in proximal and distal tubules, with less robust expression in glomeruli. All images captured at ×10 magnification. CFP, cyan fluorescent protein.