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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Arch Oral Biol. 2010 Apr 7;55(5):358–364. doi: 10.1016/j.archoralbio.2010.03.010

Figure 2.

Figure 2

Cell cycle distribution, DNA damage and p53-related changes in irradiated BMSCs. A. Cell cycle distribution of non-irradiated and irradiated BMSCs from the three skeletal sites indicate radiation-induced G0G1 arrest based on more BMSCs accumulation in G0G1 post-irradiation. B. Representative images of the comet tails visualized by SYBR Green I staining 30 minutes post-irradiation indicate more DNA damage in iliac crest relative to maxilla and mandible BMSCs (Analysis of relative changes in tail moment of ≥ 50 cells indicate statistically significant differences between IC-MSCs and OF-MSCs, P < 0.01). C and D. Representative Western blots and p53 levels based on densitometric analysis from irradiated cells (n=4 subjects) showed disparate p53 levels based on immunoreactivity to mouse anti-human p53 antibody. E. The response to radiation based on p21Waf1/Cip1 mRNA was higher in maxilla and mandible BMSCs (n=4 subjects) post-irradiation suggesting underlying mechanisms may be downstream of p53 signaling.