Fig. 1.

Primary and secondary hygromycin B hypersensitivity screens. a Strains of haploid Mat-α deletion collection were replica plated from 96-well plates onto YPD and YPD + 25 µg/ml hygromycin B plates, and were incubated for 3 days at 30°C. Rectangles depict strains hypersensitive to hygromycin B. b Putative hhy strains from the primary screen were streaked on YPD and YPD + 25 µg/ml hygromycin B plates, and were incubated for 3 days at 30°C. In this representative plate, sac1 was confirmed as an hhy mutant. env1 serves as a hypersensitive control