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State of p2CPY processing in hhy mutants. Patched cells were transferred to nitrocellulose membranes for overnight growth, and were probed with anti-proCPY-specific monoclonal antibody either without lysing cells or after lysis. Under the assay conditions, wild type has minimal signal due to efficient processing of proCPY forms into mCPY, while env1 and vps35 controls show persistent p2CPY either inside cells or secreted, respectively
