Table 2.
Strain | YPD | 1M NaCl |
1M KCl |
0.5 M KCl |
0.5 mM CaCl2 |
0.2 mM CaCl2 |
10 mM MnCl2 |
4 mM ZnCl2 |
pH 2.5 |
pH 8.0 |
20 nM rapamycin |
20 mM caffeine |
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
hhy1 | +++ | +++ | +++ | +++ | ∓ | ∓ | ∓ | ∓ | ∓ | ∓ | ∓ | ∓ |
drs2 | +++ | +++ | +++ | +++ | ++ | ++ | ++ | +++ | +++ | ++ | ∓ | ∓ |
luv1 | +++ | +++ | +++ | +++ | +++ | +++ | ∓ | ∓ | ∓ | ∓ | − | − |
sbh2 | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ∓ | ∓ | ∓ |
sac1 | +++ | ++ | +++ | +++ | +++ | +++ | +++ | ++ | ++ | ∓ | ∓ | − |
arf1 | ++ | ++ | ++ | ++ | ++ | ++ | ∓ | ++ | ++ | ++ | ++ | ∓ |
paf1 | +++ | +++ | +++ | +++ | +++ | +++ | ++ | +++ | + | ++ | − | − |
dhh1 | +++ | ++ | +++ | +++ | +++ | +++ | ++ | +++ | +++ | ++ | ∓ | − |
vps45 | +++ | ++ | +++ | +++ | +++ | +++ | ∓ | ∓ | ++ | ∓ | ∓ | − |
sac2 | +++ | ++ | +++ | +++ | +++ | +++ | ∓ | ∓ | − | ∓ | ∓ | − |
vps34 | ++ | ∓ | ++ | ++ | ++ | ++ | ++ | − | ∓ | − | − | − |
tpd3 | ++ | ∓ | + | + | + | ++ | − | − | + | ∓ | − | − |
chc1 | +++ | +++ | +++ | +++ | +++ | +++ | − | ∓ | ∓ | − | − | − |
bud32 | +++ | +++ | +++ | +++ | +++ | +++ | ∓ | ∓ | ∓ | +++ | ∓ | − |
BY4742 (WT) and 14 mutants were grown on YPD and selective media with the indicated concentrations. Following 48–96-h growth, colony number and size on plates were scored as follows: +++ strong growth; ++ moderate growth; ∓ weak growth; − no growth