Table 3.

Summary of various characterizations of hhy mutants (this study)

Gene name	Secretion	Halo assays	CPY processing	Ion/pH sensitivity	Caff./Rap. sensitivity	Vacuole morphology	Acidification	Temperature sensitivity at 37°C	Temperature sensitivity at 15°C
tpd3	Defective	Defective	WT	Yes	Yes	WT	WT	Yes	Yes
drs2	WT	WT	Defective	Slight	Yes	WT	WT	No	Yes
sbh2	WT	WT	WT	Yes	Yes	WT	WT	No	Yes
sac1	Defective	Defective	Defective	Yes	Yes	Fragmented	WT	No	Yes
arf1	WT	Defective	Defective	Yes	Yes	Fragmented	WT	No	Yes
chc1	WT	Defective	WT	Yes	Yes	WT	WT	Slight	Yes
paf1	WT	Defective	Defective	Yes	Yes	Fragmented	WT	Yes	Yes
dhh1	WT	WT	Defective	Yes	Yes	Fragmented	WT	No	Yes
bud32	WT	Defective	Defective	Yes	Yes	WT	WT	Yes	Yes
hhy1	WT	WT	Defective	Yes	Yes	Fragmented	Defective	Yes	No