. Author manuscript; available in PMC: 2011 Jun 14.

Published in final edited form as: Biomacromolecules. 2010 Jun 14;11(6):1507–1515. doi: 10.1021/bm100144v

Table 1.

Secondary Structure Analysis (infrared spectra) for the Free BSA and Its Polyamine Complexes in Hydrated Film at pH 7.4

Amide I components (cm^-1)	free BSA (%)	spermine-BSA (%)	spermidine-BSA (%)	BE-333-BSA (%)	BE-3333-BSA (%)
Amide I components (cm^-1)	0.25 mM	0.5 mM	0.5 mM	0.5 mM	0.5 mM
1692-1680 β-anti (±1%)	3	6	4	4	2
1680-1660 turn(±2%)	12	16	15	16	28
1660-1650 α-helix(±3%)	63	44	53	48	33
1648-1641 random coil (±2%)	6	22	17	20	24
1640-1610 β-sheet (±2%)	16	12	11	12	13

Experiments were repeated in 3 separate samples, and curve fitting and data analysis were performed on each experiment, and standard errors calculated. (for Tables 1 and 2)