Table 1.

Summary of real-time RT-PCR expression data for gracilis arteries from NADPH oxidase (Nox) and ANG II receptor genes in response to renal I/R injury

Unigene ID	RefSeq	Symbol	Gene Name	Log2, ratio
Rn.98491	NM_023965	Cybb-gp91	Gp91-phox	−2.80*
Rn.5856	NM_024160	Cyba-p22	p22-phox	−1.08 NS
Rn.9815	NM031009	AT1b	Angiotensin receptor, type 1b	−2.78*†
Rn.220465	NM_053683	NADPH oxidase 1	Nox1	−5.04*

Values are natural log-of-ratio-derived ΔΔCt values; negative values represent suppressed expression vs. sham-operated control.

^*Genes considered differentially expressed as defined as ratio > mean ± 2*SD using the distribution of ratios derived from the RT2 profiler array assayed identically and described in Table 2.

^†Gene had low level expression and was subsequently reassayed with 10× higher RT product than was used for all genes in Tables 1 and 2. Under these conditions, the expression was consistently detectable in the samples derived from the sham-operated control samples but not in all samples from experimental samples. For calculation purposes, the undetectable samples were considered at Ct = 40 for the computation of ΔΔCt values.