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PCR analysis of the tissue distribution of Clic5 mRNA in wild-type (WT) and Clic5 mutant (KO) mice. Total RNA was extracted from tissues of WT and KO mice and reverse transcribed, and the resulting cDNA was amplified as described in materials and methods to yield 530- and 348-bp products for WT and KO alleles, respectively. The following tissues were analyzed: 1, duodenum; 2, jejunum; 3, ileum; 4, colon; 5, stomach; 6, heart; 7, kidney; 8, liver; 9, brain.
