Fig. 6.

Plasma profile of nonfasted WT and KO mice. Plasma hormones and lipids from male WT and heterozygous mice (black circles, n = 7 WT and 4 heterozyotes) and KO mice (grey circles, n = 7) were measured without fasting. Values for WT and heterozygous mice were not significantly different, so they were pooled. Leptin levels were severely reduced in the mutant mice (0.04 ± 0.02 ng/ml), and 2 of the 7 KO mice had no detectible levels of leptin. Plasma triglycerides (TG), nonesterified fatty acids (NEFA), total cholesterol (Chol), and phospholipids (PL) were normal in the KO mice. *P < 0.05 using a Mann-Whitney rank sum test.