Table II.
Analysis of microparticle content by multiplexed sandwich ELISA.
pg/ml | IL-1b | IL-4 | IL-6 | IL-10 | IL-13 | P-selectin | IFN-γ | TNF-α | TNFRI | MMP-2 | MMP-9 |
---|---|---|---|---|---|---|---|---|---|---|---|
Wild type | 232·1 | 165·6 | 378·7 | 13·4* | 41·2 | 3476 | 844·6 | 123·7 | 9·3* | 7354* | 1924·5 |
Entpd1-null | 276·4 | 174·4 | 439·2 | 1·2 | 26·3 | 4267 | 836·7 | 189·9 | 1·9 | 1020 | 1724·4 |
Pooled MP-suspensions from wild type and Entpd1-null mice (n = 6 each) were lysed in PBS-0·1% NP-40 and then analysed for content of interleukins (IL), P-selectin, IFN-γ, interferon-gamma; TNF-α, tumour necrosis factor alpha; TNFRI, TNF receptor I; MMP, matrix metallo-proteinase.
Greater than fourfold differences between the two MP populations.