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. 2010 Jun 1;123(13):2170–2178. doi: 10.1242/jcs.066738

Fig. 7.

Fig. 7.

Bat3 relocalisation of GFP-Sed5 is independent of Sgt2. (A) Reticulocyte-lysate components eluted from recombinant TA proteins with or without a TA segment (see Fig. 2) were analysed for the presence of SGTA by immunoblotting. (B) Live-cell imaging of GFP-Sed5 in wild-type, Δsgt2 and Δmdy2sgt2 S. cerevisiae expressing full-length human Bat3. (C) Summary of potential interactions between TA proteins, Bat3, SGTA/Sgt2, Hsp70 and components of the TRC40/GET pathway (see Chang et al., 2010; Corduan et al., 2009; Costanzo et al., 2010; Favaloro et al., 2008; Jonikas et al., 2009; Sasaki et al., 2008; Schuldiner et al., 2008; Stefanovic and Hegde, 2007; Stelzl et al., 2005; Winnefeld et al., 2006). Solid lines indicate known or presumed physical interactions, the dashed line, a regulatory interaction and the dotted lines indicate possible contributions of Bat3 to TA-protein biogenesis.