Fig. 6.

Cytokinesis of cells lacking scar and mhcA. JK107 cells coexpressing HSPC300-GFP and mRFPmars-actin were grown overnight in the presence (A) or absence (B) of 10 μg/ml doxycycline and imaged by TIRF microscopy. Scale bar: 10 μm. Note the frame interval in B is twice as long as in A. (C) Cells lacking both SCAR and myosin II take significantly longer to divide. Movies were recorded of dividing cells and the time from furrow formation to abscission was measured for control (Ax3), mhcA-null (JK103), scar-null (IR46) and inducible scar/mhcA-null cells (JK107). Values plotted are the mean ± standard deviation of at least five cells. *P<0.005.