Skip to main content
. 2010 Jun 8;123(13):2332–2341. doi: 10.1242/jcs.069443

Fig. 3.

Fig. 3.

Twist promotes tumor cell extravasation by increasing tumor cell intravascular migration and membrane-protrusion dynamics dependent on ROCK kinase activity. (A) Comparison of membrane protrusion dynamics of MDAtwist and MDAwt cells. Middle panel, red and blue channels showing individual tumor cell shapes. Right panel shows schematic outlines of the blood vessel and MDAtwist tumor cell. (B) Intravascular shape index (sphericity, round=1.0) for individual MDAtwist and MDAwt cells for the indicated times. Each line in B represents relative change in sphericity over the indicated time period for individual MDAwt or MDAtwist tumor cells. (C) Average speed of shape index change for MDAwt and MDAtwist cells. (D) Effect of ROCK and MLCK inhibition on Twist-induced tumor cell extravasation. (E) β1 integrin expression on MDAwt and MDAtwist cells (MFI, mean fluorescence intensity units) as determined by FACS analysis. (F) Average numbers of MDAwt and MDAtwist cells attached to various extracellular matrixes (3 hour time point) per 40× field. Results are means ± s.e.m. Scale bars, 20 μm.