APS is retained in permeabilized detrusor smooth muscle (DSM). When subjected to the protocol shown in A, for each muscle length, the tension value representing an estimate of APS (TLoad − TUnload) obtained during round 1 Load and UL was greater than that produced during round 2 (B). When subjected to the protocol shown in C, for each muscle length, the tension value representing an estimate of APS (TLoad − TUnload) obtained during round 1 Load and UL was equal to that produced during round 2 (D). SS, strain softening. Data in B and D are means ± SE, n = 3. *P < 0.05 compared with round 1, Student's t-test.