Table 1. The genotype ratio was distorted after IVF with oocytes from Shb +/− mothers and Shb +/+ sperm.

Stage	Shb +/+	Shb +/−
2–8 cell stage	1* (7%)	13 (93%)
Morula	9 (36%)	16 (64%)
Unfertilized	15 (31%)	34 (69%)
Degenerated	7 (21%)	27 (79%)

Oocytes from +/− mothers were isolated, in vitro fertilized with +/+ sperm and differentiated in vitro as in Fig. 6. At 24 h after IVF the early embryos/oocytes were classified and genotyped. The absolute and relative (in percent) +/+ vs +/− embryos/oocytes are given for each category. * indicates p<0.05 when compared with the corresponding number of morulas using the Chi-square test. The total percentage +/+ genotype was 26% for 122 observations.