Table 4. Microsatellite markers of Acropora species used in this study.
| Locus | Repeat motif | Primer sequence (5′-3′) | Size range of alleles (nt) | Multiplex and primers concentration (nM) | Reference |
| MS166 | (AAT)2AAAAATAAC(AAT)4 | D3 (green)-TCTACCCGCAATTTTCATCA | 116–160 | multiA | Baums et al. [16] |
| CGCTCTCCTATGTTCGATTG | 40 (F: 20, R: 20) | ||||
| MS181 | (AAT)5GAT(AAT)5ATT(AAT)3 | D4 (blue)-TTCTCCACATGCAAACAAACA | 143–269 | multiA | Baums et al. [16] |
| GCCAGGATAGCGGATAATGA | 60 (F: 30; R: 30) | ||||
| MS182 | (AAT)10 | D4 (blue)-TCCCACAACTCACACTCTGC | 128–231 | multiB | Baums et al. [16] |
| ACGCGGAAATAGTGATGCTC | 46 (F: 23; R: 23) | ||||
| MS8 | (CT)3GT(CT)5 | D3 (green)-GATCCGTCACACTTGTTCTAAGG | 80–91 | multiB | Nakajima et al. [27] |
| TGACTGTCAGAGTAGAGGGAAGG | 54 (F: 27; R: 27) | ||||
| A.mill2-8 | (AC)6 | D2 (black)- AGGTTTCTATGGGAACGTCG | 90–96 | multiC | van Oppen et al. [18] |
| TGAACTTCAAGTAATTTTGCCAG | 50 (F: 25; R: 25) | ||||
| A.mill2-22 | (AC)10 | D4 (blue)-CTGTGGCCTTGTTAGATAGC | 158–192 | multiC | van Oppen et al. [18] |
| AGATTTGTGTTGTCCTGCTT | 50 (F: 25; R: 25) |
D2 (black), D3 (green), and D4 (blue) in the primer sequence are fluorescent dye labels (Sigma-Genosys, St. Louis, MO, USA). The size range was suggested by analysis using a DNA sequencer (CEQ-8800; Beckman Coulter, Fullerton, CA, USA). Two capitals in the column multiplex and primers concentration represent forward (F) and reverse (R), respectively.