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. 2010 Jun 16;5(6):e11056. doi: 10.1371/journal.pone.0011056

Figure 5. In vitro effecs of rhGH on hematopoietic stem/progenitor cells.

Figure 5

Irradiated (3 Gy [C,D] or 4 Gy [A,B]) c-Kit+Lin-Sca-1+ cells from C57BL/6 mice were cultured with thrombopoietin, stem cell factor, Fl3 ligand and various concentration of recombinant human growth hormone. In some experiments shown in C and D, osteoblasts were added to the culture. After 24 hours in culture, percentages of apoptotic (A) and dead (B) cells among c-Kit+Lin−Sca-1+ hematopoietic stem/progenitor cells were determined by flow cytometry. Similar results were obtained at 48 and 72 hours. In the experiments shown in C and D, the cells were harvested after 7 days in culture. The frequencies of hematopoietic stem and progenitor cells were then determined by in vitro colony forming unit assay (C) and in vivo CFU-S assay (D) respectively. *P<0.05, **P<0.01, ***P<0.001. rhGH stands for recombinant human growth hormone; TSF, thrombopoietin, stem cell factor, Fl3 ligand, CFU-S, colony forming unit-spleen; CFC, colony forming cell.