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Immunohistochemical analysis showing expression and immunolocalization of (a) clusterin, (b) Kim-1, (c) Lcn2, (d) vimentin, and (e) Timp-1 in kidneys of male Wistar rats treated with BI-2, BI-3, or gentamicin. Representative images from cortical regions of controls (insets) and high-dose animals after 14 days of treatment (3 days for BI-3 and Lcn2) are shown. Images were acquired at ×200 magnifications using an Olympus CH-2 microscope fitted with an Olympus E330 digital camera.
