FIG. 5.

Effect of cadmium on CFTR mRNA transcript levels in Calu-3 cells. (A) Calu-3 cells were incubated with 50μM cadmium for 4–48 h. (B) Calu-3 cells were incubated for 24 h with 10–100μM cadmium. (C) qRT-PCR analysis of ZIP8 mRNA transcript levels were analyzed after treatment of Calu-3 cells with 50μM cadmium for 24 h. CFTR and ZIP8 mRNA transcript levels were measured as described in “Materials and Methods” section. Data are expressed as mean ± SEM of at least three independent experiments.