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The heavy metal nickel and nornicotine (a metabolite of nicotine) have no effect on CFTR protein. (A) The human bronchial epithelial cells Calu-3 were incubated for 3 days with nickel (50μM). Data are expressed as mean ± SEM. Number of experiments (n). (B) Calu-3 cells were incubated with 50μM cadmium or 10 and 100μM nornicotine for 3 days. CFTR protein was detected as described in the “Materials and Methods” section. Data are representative of at least three independent experiments.
