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The antioxidant NAC does not prevent cadmium-induced reduction of CFTR protein in Calu-3 cells. Calu-3 cells were incubated for 3 days with 50μM cadmium (Cd) alone or in combination with NAC (10mM). CFTR was detected using anti-C-CFTR antibody (24-1; R and D Systems) as described in the “Materials and Methods” section. **p < 0.001.
