Figure 1.

(A) Agarose gel of typical PCR amplification patterns of the nested PCR products of the DNA from P falciparum-infected blood samples for PFCRT gene, showing the expected product of 154 bp. M: 100-bp ladder, lanes 1 and 2: no-template negative controls, lanes 3-6: PCR product from representative patient samples. (B) Patterns obtained after restriction digestion of the PCR products with ApoI. Lane 1: restriction digestion control, lanes 2-5: PCR product from representative patient samples after digestion with ApoI. (C) Chromatogram confirming the presence of mutation at the 76th amino acid position highlighted in yellow