Figure 2.

(A) Agarose gel of typical PCR amplification patterns of the nested PCR products of the DNA from P falciparum-infected blood samples for PFMDR1 gene, giving the expected product of 560 bp. M: 100-bp ladder, lanes 1 and 2: no-template negative controls, lanes 3-6: PCR product from representative patient samples. (B) Patterns obtained after restriction digestion of the PCR products with ApoI. The products of the wild-type allele are 250 bp, 231 bp and 79 bp, while, two fragments of 481 bp and 97 bp are evident for the mutant alleles. M: 100-bp ladder, lanes 1: undigested PCR product, lanes 2-5: PCR product from representative patient samples after digestion with ApoI. (C and D) representative chromatograms confirming the presence of mutant and wild alleles at the 86th amino acid position.