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. 2010 May 6;41(1):73–80. doi: 10.1007/s11262-010-0488-1

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© The Author(s) 2010

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Fig. 1 — VLP formations from the DGNNV capsid protein with cysteine mutations. a The intensity profiles of wild-type and cysteine mutants determined by sucrose density gradient centrifugation. Bold line: WT (no mutation); thin line: C115A; dotted line: C201A. b Bold line: C331A; thin line: C187A; dotted line: C187A/C331A. Fractions from #5 to #15 are denoted as I-f5-15 and fraction #24 is denoted as II-f24. c, d Micrographs from fractions #5 to #15 and fraction #24 of the cysteine mutants. The arrow indicates the broken particles with irregular shape. e Control: native DGNNV virus. Bar: 50 nm