Fig. 1.

Expression of SARS-CoV and NL63 S proteins using recombinant baculoviruses. Western blots of expression at 2 days p.i. with viruses encoding proteins either as Fc fusion proteins (a) or following fusion to the VSV G TM domain (b). In (a), supernatant was probed with anti-human Ig; in (b), the cell pellet was probed with anti-VSV G protein. Lanes: 1, SARS-CoV S; 2, SARS-CoV S1 (residues 19–713); 3, NL63 S; 4, NL63 S_15–195; 5, NL63 S_15–739; 6, NL63 S_196–739. Molecular mass markers are indicated (kDa).