. Author manuscript; available in PMC: 2011 May 17.

Published in final edited form as: Chem Res Toxicol. 2010 May 17;23(5):986–995. doi: 10.1021/tx1000943

Table 2.

Analysis of Linoleate HODE, HETEs and Isoprostanes in Mouse Brain

	BHT, NaBH₄	BHT, NaBH₄ 100μM EDTA	BHT, NaBH₄ 1 mM EDTA	BHT, PPh₃	BHT, PPh₃ 100μM EDTA	BHT, PPh₃ 1 mM EDTA
Mouse brain (HODE, HETE, isoPs: ng/mg tissue)
9ZE HODE	0.49 ± 0.1	0.56 ± 0.03	0.54 ± 0.05	0.51 ± 0.02	0.49 ± 0.004	0.49 ± 0.01
9EE HODE	0.25 ± 0.1	0.18 ± 0.04	0.14 ± 0.01	0.028 ± 0.005	0.047 ± 0.01	0.039 ± 0.005
13 ZE HODE	0.43 ± 0.2	0.54 ± 0.07	0.49 ± 0.06	0.55 ± 0.07	0.56 ± 0.07	0.54 ± 0.07
13 EE HODE	0.21 ± 0.1	0.11 ± 0.03	0.069 ± 0.01*	0.0054 ± 0.004	0.0056 ± 0.004	0.015 ± 0.008
ZE HODE	0.92 ± 0.3	1.1 ± 0.1	1.0 ± 0.1	1.1 ± 0.06	1.0 ± 0.08	1.0 ± 0.06
EE HODE	0.46 ± 0.1	0.29 ± 0.07	0.21 ± 0.02*	0.033 ± 0.007	0.053 ± 0.01	0.054 ± 0.01
ZE/EE	2.2 ± 1	4.0 ± 1	5.0 ± 0.9	32 ± 5	21 ± 6	19 ± 3*
t-HODE	1.4 ± 0.2	1.4 ± 0.04	1.2 ± 0.09	1.1 ± 0.07	1.1 ± 0.07	1.1 ± 0.06
t-HETE	8.3 ± 0.2	7.8 ± 0.7	7.5 ± 0.8	11 ± 0.2	12 ± 2	13 ± 2
IsoPs	0.44 ± 0.3	0.26 ± 0.04	0.27 ± 0.03	0.091 ± 0.01	0.079 ± 0.02	0.083 ± 0.009