Fig. 5.
Quantitative RT-PCR analysis of IDEF2 (A) and its typical target genes OsYSL2, AK065090, AK099523 and AK103890 (B) under Fe-rich and early Fe-deficient conditions in hydroponic culture. IDEF2-RNAi transformants (lines 22 and 23, T1) and NT were either supplied with 15·8 mg L−1 Tetsuriki-TypeX fertilizer (Fe-rich) or cultured in Fe-free solution (–Fe 1 d) for 24 h. Transcript abundances in roots were quantified and are expressed as ratios relative to NT levels in Fe-rich conditions (mean ± s.d.; n = 3–6). Significant differences from NT, analysed using a t-test (*P < 0·05; **P < 0·01), are shown.