Fig. 7.

IEF/SDS–PAGE resolution of water-soluble chloroplastic proteins of the fourth (top) and sixth (bottom) rice leaf without (left) and with (right) 50 µm Mn supply for 3 d. Chloroplasts were isolated using an adapted method described originally by Tanaka et al. (2004). After purification, chloroplasts were frozen in liquid nitrogen. After subsequent thawing of the chloroplasts, thylakoids were centrifuged. The supernatant was precipitated and finally used for electrophoresis. After separation, gels were stained with silver nitrate. Comparative visual inspection of the resolutions led to the identification of at least seven differentially affected proteins in the younger leaf (marked with red arrows).