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Representative Coomassie-stained 2D IEF/SDS–PAGE resolution of the proteome of the older fourth (top) and the younger sixth (bottom) rice leaf blades after elevated Mn supply (50 µm) for 3 d (right) or continuous 1 µm Mn supply (left). Three biological replications of each treatment were analysed using the Imagemaster™ 2D PLATINUM Software 6·0. Significantly regulated (P < 0·05) protein spots (marked by arrows) were cut out and identified by nano LC-MS/MS.
